Composite

Part:BBa_K2839009

Designed by: Grigorios Kyrpizidis   Group: iGEM18_Thessaloniki   (2018-10-08)


pRha-sgRNA Cassette test construct without reporter

2.1 sgRNA cassette

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 235
    Illegal NheI site found at 504
    Illegal NheI site found at 527
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 646


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Categories
Parameters
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